Primary Metabolism by GC-TOF-MS

carbohydrates and sugar phosphates, amino acids, hydroxyl acids, free fatty acids, purines, pyrimidines, aromatics, exposome-derived chemicals


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For blood plasma, urine, fecal matter, and tissue samples of various origins samples, normalized relative intensities are reported for up to 200 identified metabolites in addition to 200-300 compounds of unknown chemical structure.

Samples from other sources (tissues, cell cultures) may differ in the number of identified and unidentified compounds.

All metabolites are reported by retention index, quantification mass, biochemical database identifiers and full mass spectra. Metabolites are quantified by peak heights, not in absolute concentrations. However, we can add internal standards and use calibration curves as needed to give absolute (micromolar) concentrations as requested, e.g. for TCA (Krebs cycle) intermediates, glycolysis, pentose phosphate pathway metabolites, amino acids or other targets! Through untargeted analyses, we also detect exposome-type compounds, from microbial conversions of food metabolites to pharmaceutical drugs.