flavonoids, anthocyanins, polyphenols, alkaloids, coumarins, terpene- and polyphenol-glycosides, kaempferols, flavanones, cinnamates, and related compounds
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- For plant, food and gut intestinal/fecal matter samples of various origins, normalized relative intensities are reported for up to 400 identified polyphenols and natural products in addition to a large list of unknowns. These compounds are identified by matching mass spectra in a targeted library of MS/MS spectra in addition to accurate masses and retention time matching.
- Untargeted data processing may add a large number of additional metabolites of unknown chemical structure.
- All polyphenols and natural products are reported by retention times, quantification masses, biochemical database identifiers and adduct information. These natural products are quantified by peak heights, not in absolute concentrations. However, we are adding 10 internal standards to convert peak heights into good estimates of absolute (micromolar) concentrations for a range of polyphenols typically detected in foods or plant tissues! Through untargeted analyses in urine, we may also detect exposome-type compounds, from polyaromatic hydrocarbons to PCBs, using glucuronidase in sample pre-treatment.