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Equipment

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Equipment

Together with the adjacent Fiehn metabolomics research laboratory, the facility currently hosts 14 mass spectrometers (7 GC/MS and 7 LC/MS instruments), including five accurate mass instruments.  Overall, more than 30 mass spectrometers and 5 NMR instruments are available for comprehensive analysis of metabolism in the NIH West Coast Metabolomics Center.

 

10 LCMS instruments

2 Agilent 6530 QTOF mass spectrometers with 1290 UHPLC
1 Agilent 6550 QTOF mass spectrometer with 1290 UHPLC
2 Sciex QTRAP (6500, 4000
) mass spectrometers with Waters UPLC
1 Sciex Triple TOF mass spectrometer with Eksigent microLC
2 ThermoScientific ion trap LTQ
mass spectrometers with Advion NanoMate robotic nanoelectrospray or 1290 UHPLC

currently on loan, testing:

1 Agilent 6560 ion mobility QTOF mass spectrometer with 1290 UHPLC
1 ThermoScientific Q-Exactive Plus orbitrap mass spectrometer with ThermoScientific UHPLC

7 GCMS instruments

4 LECO Pegasus IV GC-time of flight mass spectrometers with Gerstel automatic liner exchange and cold injection system,
plus Gerstel Twister thermodesorption unit for profiling of volatile compounds, plus GCxGC option
1 Agilent GC-QTOF
mass spectrometer for chemical or electron ionization
2 Agilent GC-quadrupole mass spectrometers with autosampler and s/sl injector or APEX large volume injector

5 NMR instruments

2x 600 and 800 MHz Bruker Avance III spectrometers with cryoprobes.

1x 500 MHz Bruker Avance DRX spectrometer with a 5 mm 1H/BB probe with an X-channel tunable from 15N to 31P. This spectrometer is also capable of both low-temperature and high-temperature experiments, and has an automatic sample changer for high-throughput work.

2x spectrometers capable of MRI / Imaging. The 400 MHz Bruker DRX spectrometer is a vertical-bore instrument with both spectroscopy and microimaging capabilities, and has both Topspin and Paravision 4 software for spectrometer control. The 7T (300 MHz) MRI instrument is a horizontal-bore spectrometer ideal for small-animal imaging.

Computational and Chemical Resources

The Metabolomics facility is supported by dedicated servers, tools and databases, including a 200 TB mirrored server for raw data storage. The facility stores over 3,000 unique metabolite standards. A range of -80°C and -20°C freezers in addition to grinders, homogenizers, sonicators, incubators, shakers and mixers are available for sample storage and processing.

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