Together with the adjacent Fiehn metabolomics research laboratory, the facility currently hosts 14 mass spectrometers (7 GC/MS and 7 LC/MS instruments), including five accurate mass instruments. Overall, more than 30 mass spectrometers and 5 NMR instruments are available for comprehensive analysis of metabolism in the NIH West Coast Metabolomics Center.
10 LCMS instruments
2 Agilent 6530 QTOF mass spectrometers with 1290 UHPLC
1 Agilent 6550 QTOF mass spectrometer with 1290 UHPLC
2 Sciex QTRAP (6500, 4000) mass spectrometers with Waters UPLC
2 ThermoScientific ion trap LTQ mass spectrometers with Advion NanoMate robotic nanoelectrospray or 1290 UHPLC
currently on loan, testing:
1 Agilent 6560 ion mobility QTOF mass spectrometer with 1290 UHPLC
1 ThermoScientific Q-Exactive Plus orbitrap mass spectrometer with ThermoScientific UHPLC
7 GCMS instruments
4 with mass spectrometersGerstel automatic liner exchange and cold injection system,
plus Gerstel Twister thermodesorption unit for profiling of volatile compounds, plus GCxGC option
1 Agilent GC-QTOF mass spectrometer for chemical or electron ionization
2 Agilent GC-quadrupole mass spectrometers with autosampler and s/sl injector or APEX large volume injector
5 NMR instruments
2x 600 and 800 MHz Bruker Avance III spectrometers with cryoprobes.
1x 500 MHz Bruker Avance DRX spectrometer with a 5 mm 1H/BB probe with an X-channel tunable from 15N to 31P. This spectrometer is also capable of both low-temperature and high-temperature experiments, and has an automatic sample changer for high-throughput work.
2x spectrometers capable of MRI / Imaging. The 400 MHz Bruker DRX spectrometer is a vertical-bore instrument with both spectroscopy and microimaging capabilities, and has both Topspin and Paravision 4 software for spectrometer control. The 7T (300 MHz) MRI instrument is a horizontal-bore spectrometer ideal for small-animal imaging.
Computational and Chemical Resources
The Metabolomics facility is supported by dedicated servers, , including a 200 TB mirrored server for raw data storage. The facility stores over 3,000 unique metabolite standards. A range of -80°C and -20°C freezers in addition to grinders, homogenizers, sonicators, incubators, shakers and mixers are available for sample storage and processing.